Distribution of Lipase in Milk Proteins. I. DEAE Cellulose Column Chromatography

Separation of folate binding protein from human serum by DEAE-cellulose column chromatography.

On diethylaminoethyl-cellulose column chromatography, the folate binding protein in the serum of 21 patients eluted in the early effluents as a single sharply defined peak. The chromatographic behavior of the folate binder remained unchanged whether or not the serum was, before chromatography, complexed with tritium-labeled pteroylglutamic acid ([3H]PGA), dialyzed, or charcoal-adsorbed. Heating...

Gel electrophoretic analysis of peanut proteins and enzymes. I. Characterization of DEAE-cellulose separated fractions.

Biliary alkaline phosphatase measured by mini-column chromatography on DEAE-cellulose: application to detection of hepatobiliary diseases.

Using chromatography on diethylaminoethyl (DEAE) cellulose, we measured biliary alkaline phosphatase (BALP; EC 3.1.3.1) activities in sera from 182 patients, most with hepatobiliary disorders but some with non-hepatobiliary diseases. Relative BALP activities were extremely low in otherwise healthy carriers of hepatitis B virus (mean: 5.4 U/L) and in patients with non-hepatobiliary diseases (mea...

Separation of vitamin B 12-binding proteins of serum, gastric juice and saliva by rapid DEAE cellulose chromatography.

By FRANCOIS P. RETIEF, CHESTER W. GOTFLIEB, SHAUL KOCHWA, PETER W. PRATF AND VICTOR HERBERT N ORMAL SERUM contains at least two binders of vitamin B12, one migrating electrophoretically as an alpha-globulin2’3 and the other as a beta-globulin.46 There is evidence that these substances have different metabolic functions. Whereas native B12 is bound to alpha-globulin,2 the betaglobulin binder pro...

Analytical separations of metals by column chromatography on microcrystalline cellulose